Solution - Bioprocess Engineering Basic Concepts 2nd Edition

1.1. What are the main goals of bioprocess engineering?

2.1. A bioreactor contains 1000 L of medium with an initial cell concentration of 1 g/L. If 500 L of medium is added, what is the new cell concentration? Bioprocess Engineering Basic Concepts 2nd Edition Solution

Solution: Power per unit volume = 2 kW / 2000 L = 0.001 kW/L or 1 W/L. A bioreactor contains 1000 L of medium with

5.1. A medium is sterilized at 121°C for 15 minutes. If the initial number of spores is 10^6 per mL and the death rate constant is 0.5 min^-1, what is the final number of spores per mL? Bioprocess Engineering Basic Concepts 2nd Edition Solution

Solution: Mass of medium = 1000 L * 1000 g/L = 1,000,000 g or 1000 kg. Energy required = 1000 kg * 4.2 kJ/kg°C * (37°C - 20°C) = 1000 * 4.2 * 17 = 71,400 kJ.

1.1. What are the main goals of bioprocess engineering?

2.1. A bioreactor contains 1000 L of medium with an initial cell concentration of 1 g/L. If 500 L of medium is added, what is the new cell concentration?

Solution: Power per unit volume = 2 kW / 2000 L = 0.001 kW/L or 1 W/L.

5.1. A medium is sterilized at 121°C for 15 minutes. If the initial number of spores is 10^6 per mL and the death rate constant is 0.5 min^-1, what is the final number of spores per mL?

Solution: Mass of medium = 1000 L * 1000 g/L = 1,000,000 g or 1000 kg. Energy required = 1000 kg * 4.2 kJ/kg°C * (37°C - 20°C) = 1000 * 4.2 * 17 = 71,400 kJ.

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